竹节蓼药材的HPLC指纹图谱建立及聚类分析

发布时间:2019-08-30 来源: 历史回眸 点击:


  中图分类号 R927 文献标志码 A 文章编号 1001-0408(2018)12-1640-04
  DOI 10.6039/j.issn.1001-0408.2018.12.13
  摘 要 目的 建立竹节蓼药材的高效液相色谱(HPLC)指纹图谱,并进行聚类分析。方法:采用HPLC法。色谱柱为Ultimate C18,流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为0.5 mL/min,检测波长为360 nm,柱温为28 ℃,进样量为10 μL。以槲皮苷为参照,绘制13批药材样品的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2012版)进行相似度评价,确定共有峰,并对其进行聚类分析。结果:13批药材样品的HPLC图谱有12个共有峰,相似度均大于0.90;经验证,13批药材样品的HPLC图谱与对照指纹图谱具有较好的一致性。13批药材样品可聚为两大类,S4、S6、S10聚为一类,其余聚为一类。结论:该研究所建指纹图谱和聚类分析结果可为竹节蓼药材的质量评价及进一步开发利用提供参考。
  关键词 竹节蓼;高效液相色谱法;指纹图谱;聚类分析
  ABSTRACT OBJECTIVE: To establish HPLC fingerprint of Homalocladium platycladum, and to carry on the cluster analysis. METHODS: HPLC method was adopted. The determination was performed on Ultimate C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid (gradient elution) at the flow rate of 0.5 mL/min. The detection wavelength was set at 360 nm, and column temperature was 28 ℃. The sample size was 10 μL. Using quercetin as reference substance, HPLC chromatograms of 13 batches of samples were determined. The similarity evaluation was carried out by using Similarity Evaluation System for TCM Chromatographic Fingerprint (2012 edition) to determine common peak. Cluster analysis were also conducted. RESULTS: A total of 12 common peaks were established in HPLC fingerprints of 13 batches of samples. The similarity was higher than 0.90. After validation, HPLC chromatograms of 13 batches of sample were in good agreement with control fingerprint. 13 batches of medicinal materials were clustered into 2 categories; S4, S6 and S10 were clustered into one category, and the rest were a category. CONCLUSIONS: Established fingerprint and cluster analysis results can provide reference for quality evaluation of H. platycladum and its exploitation and utilization.
  KEYWORDS Homalocladium platycladum; HPLC; Fingerprint; Cluster analysis
  竹節蓼又名观音竹、飞天蜈蚣、扁竹[1-2],其味甘、淡,性平,归肝、肺经,具有清热解毒、去瘀消肿之功效,主治痈疮肿毒、跌打损伤等症[3]。壮医认为其味甘、酸,性微寒,具有清热解毒、活血止血的功效,临床主要用于扁桃体炎、支气管炎、尿路炎症及崩漏带下、痈疮肿毒、跌打损伤、蛇虫咬伤等病症的治疗[4]。目前,竹节蓼的研究主要集中在抗肿瘤[5-7]、抗菌[8-9]作用和活性组分等方面,而在质量评价方面研究较少。本课题组前期已通过高效液相色谱法(HPLC)测定了其中槲皮苷的含量[10],在此基础上,本试验采用HPLC法对广西5个不同产地13批竹节蓼药材展开指纹图谱研究,并进行聚类分析,以期为其质量评价及进一步开发利用提供参考。
  1 材料
  1.1 仪器
  1100型HPLC仪,包括G-1314A单波长检测器、G-1311A高压四元泵、G-1379A在线真空脱气机、G-1316A智能化柱温箱、G-1311A标准自动进样器、1100 Series色谱工作站(美国Agilent公司);FA1104型上皿天平(上海精密科学仪器有限公司);KQ5200B型超声波清洗器(昆山市超声仪器有限公司);DHG-9070A型电热鼓风干燥箱(上海一恒科学仪器有限公司);HH-S6型电热恒温水浴锅(金坛市医疗仪器厂);TGT-16G型高速台式离心机(上海安亭科学仪器厂)。
  1.2 试剂

相关热词搜索:竹节 图谱 指纹 药材 建立

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